Subject: Chemistry
Book: General Chemistry
The Beer-Lambert law (A = εlc) states that absorbance (A) of a solution is directly proportional to the path length (l) and the concentration (c) of the absorbing species, where ε is the molar absorptivity. This relationship underpins UV-Vis spectroscopy, allowing quantitative analysis of solutions by measuring absorbance at specific wavelengths. Accurate application requires careful instrument calibration and reagent handling.
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